Dissociation of Amino Acid Acceptor Function of Srna from Its Transfer Function.
نویسندگان
چکیده
It is now established that the biological function of transfer RNA (sRNA) is to accept amino acid at a terminus of the chain, resulting in the formation of aminoacylsRNA,1-3 and to transfer the amino acid from that aminoacyl-sRNA into a polypeptide chain by interacting with messenger RNA on the surface of polyribosomes.4, I A number of indirect observations6-9 suggest that the sites for these reactions are located independently in the sRNA molecule. Chemical'0-'3 and enzymatic14' 15 modifications of the sRNA have provided the most direct information on this problem. We previously reported that sRNA could be altered enzymatically without loss of acceptor activity for some amino acids when it was incubated with B. subtilis RNase or RNase T, in the presence of Mg++."S. 16 The present report shows that these treated sRNA's cannot transfer certain amino acids into protein, although they still can accept these amino acids, a result indicating clearly that the amino acid acceptor function is not identical with the transfer function of sRNA. It is also shown that the amino acid acceptor activities of enzymatically altered sRNA's are inactivated by increasing temperature at low concentrations of Mg++, and the extent of this inactivation seems to be correlated with the inability of the treated sRNA to transfer amino acids. The preliminary studies for the present investigation were reported previously.'5
منابع مشابه
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 53 شماره
صفحات -
تاریخ انتشار 1965